|
| Status |
Public on May 23, 2018 |
| Title |
Multiscale analysis of a regenerative therapy for treatment of volumetric muscle loss injury |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Skeletal muscle possesses a remarkable capacity to regenerate when injured, but when confronted with major traumatic injury resulting in volumetric muscle loss (VML), the regenerative process consistently fails. The loss of muscle tissue and function from VML injury has prompted development of a suite of therapeutic approaches but these strategies have proceeded without a comprehensive understanding of the molecular landscape that drives the injury response. Herein, we administered a VML injury in an established rodent model and monitored the evolution of the healing phenomenology over multiple time points using muscle function testing, histology, and expression profiling by RNA sequencing. The injury response was then compared to a regenerative medicine treatment using orthotopic transplantation of autologous minced muscle grafts (~1 mm3 tissue fragments). A chronic inflammatory and fibrotic response was observed at all time points following VML. These results suggest that the pathological response to VML injury during the acute stage of the healing response overwhelms endogenous and therapeutic regenerative processes. Overall, the data presented delineate key molecular characteristics of the pathobiological response to VML injury that are critical effectors of effective regenerative treatment paradigms.
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| |
|
| Overall design |
RNA-Seq time couse of muscle volumetric muscle loss injury healing with controls
|
| |
|
| Contributor(s) |
Ricke DO, Aguilar C |
| Citation(s) |
29531830 |
| Submission date |
May 22, 2018 |
| Last update date |
Oct 22, 2018 |
| Contact name |
Darrell Orlyn Ricke |
| E-mail(s) |
Darrell.Ricke@ll.mit.edu
|
| Phone |
1-781-981-8323
|
| Organization name |
MIT Lincoln Laboratory
|
| Department |
Group 48 Bioengineering Systems & Technology
|
| Lab |
Bioinformatics
|
| Street address |
244 Wood Street
|
| City |
Lexington |
| State/province |
MA |
| ZIP/Postal code |
02421-6426 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (138)
|
|
| Relations |
| BioProject |
PRJNA472641 |
| SRA |
SRP148772 |
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