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| Status |
Public on Jun 06, 2018 |
| Title |
RNA-seq Analysis of the effect of Tfcp2l1 overexpression on Human ESC maintenace |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose:The goals of this study are to analyze the candidate genes motified by Tfcp2l1 overexpression to regulated the human embryonic stem cell (ESC) self-renewal through RNA-seq approach.
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| Overall design |
HES2 human ESCs transfected with Piggybac(PB) or PB-Tfcp2l1 plasmid were cultured in N2B27/10% KSR supplemented with 10 ng/ml FGF2 and 10 ng/ml Activin A for three passages and then were collected by using Trizol. These samples were sent to BGI (China) company to do deep sequencing by using BGISEQ-500.
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| Contributor(s) |
Ye S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 30, 2018 |
| Last update date |
Jan 07, 2019 |
| Contact name |
shoudong ye |
| Organization name |
Anhui University
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| Department |
Cell Biology
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| Street address |
No 111, Jiu Long Road
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| City |
Hefei |
| ZIP/Postal code |
230601 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA473659 |
| SRA |
SRP149318 |
