|
| Status |
Public on Oct 30, 2018 |
| Title |
Determination of branch point (BP) usage by wild-type and mutant SF3B1 using a minigene library |
| Organisms |
Homo sapiens; Mus musculus; synthetic construct |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We address define differential usage of branchpoint (BP) in by wild-type SF3B1 SF3B1-K700E using a synthetic mini-gene synthetic library with variable 3' spice sites (3'SS). Minigene-specific libraries were prepared and sequenced on the illumina platform to determine differnces in splice-site usage.
|
| |
|
| Overall design |
Minigene-based analysis of differential splicing
|
| |
|
| Contributor(s) |
Pillai MM, Paul K, Gupta AK |
| Citation(s) |
30462273 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL098608 |
Cyclin E Regulation in Normal and Neoplastic Hematopoiesis |
BloodCenter of Wisconsin |
Alexander C Minella |
| R01 HL104070 |
Role of microRNAs in regulation of the marrow microenvironment |
YALE UNIVERSITY |
Manoj M. Pillai |
| R01 HL133406 |
Deregulation of SF3B1 function by recurrent HEAT domain mutations |
YALE UNIVERSITY |
Manoj M. Pillai |
|
| Submission date |
Jun 10, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
MANOJ PILLAI |
| E-mail(s) |
MANOJ.PILLAI@YALE.EDU
|
| Organization name |
YALE UNIVERSITY
|
| Lab |
PILLAI LAB
|
| Street address |
300 GEORGE STREET #786
|
| City |
NEW HAVEN |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL15228 |
Illumina HiSeq 2000 (synthetic construct) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (7)
|
| GSM3181838 |
293T_3'SS library_SF3B1-WT_RNA-seq_ALL |
| GSM3181839 |
293T_3'SS library_SF3B1-WT_RNA-seq_selected |
| GSM3181840 |
293T_3'SS library_SF3B1-K700E_RNA-seq_ALL |
| GSM3181841 |
293T_3'SS library_SF3B1-K700E_RNA-seq_selected |
| GSM3181842 |
mESC_3'SS library_SF3B1-K700K_RNA-seq_ALL |
| GSM3181843 |
mESC_3'SS library_SF3B1-K700E_RNA-seq_ALL |
| GSM3181844 |
3'SS plasmid DNA library_seq |
|
| Relations |
| BioProject |
PRJNA475417 |
| SRA |
SRP150151 |
Less...
More...