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| Status |
Public on Jan 12, 2019 |
| Title |
Transcriptional profiling by 4SU-seq in mouse ESCs and ESC-derived neural progenitor cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nascent RNA was metabolically labelled with 4SU in undifferentiated and ESC-derived neural progenitor cells (NPCs). 4SU incorporated RNA was isolated and deep-sequenced at day 0 (ESCs), 3, 5 and 7 of differentiation. NPC differentiation was monitored through expression of a GFP reporter insereted into the Sox1 locus (46C reporter ESC line; PMID: 12524553). The aim was to monitor changes in transcription as ESCs differentiate into NPCs and relate this to enhancer activity.
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| Overall design |
For each of the 4 differentiation time points, two independent biological replicates were prepared and sequenced. For each assayed time point, both merged and individual replicate 4SU-seq profiles were generated.
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| Contributor(s) |
Benabdallah NS, Williamson I, Illingworth RS, Boyle S, Sengupta D, Therizols P, Bickmore WA |
| Citation(s) |
31494034 |
| Submission date |
Jun 13, 2018 |
| Last update date |
Jun 19, 2020 |
| Contact name |
Rob S Illingworth |
| E-mail(s) |
robert.illingworth@ed.ac.uk
|
| Phone |
01316519640
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| Organization name |
The University of Edinburgh
|
| Department |
Centre for regenerative Medicine
|
| Lab |
Illingworth
|
| Street address |
Centre for Regenerative Medicine
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE89557 |
A new model for long-range chromatin reorganization linked to enhancer activation |
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| Relations |
| BioProject |
PRJNA475990 |
| SRA |
SRP150459 |
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