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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 24, 2019 |
| Title |
Nuclear miCLIP and Nuclear NKAP iCLIP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify m6A sites on endogenous nuclear RNAs, we performed miCLIP to identify m6A sites in PANC-1 cells. To identify NKAP binding sites on endogenous nuclear RNAs, we performed iCLIP for flag-tag NKAP to analyze the nuclear RNA binding with NKAP in the same cells.
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| Overall design |
PRJNA477937;SRP151346;PANC-1 cells overexpressing FLAG-tag NKAP were subjected to the iCLIP procedure on immunoprecipitated NKAP associated RNA obtained from the nuclear fraction (Licatalosi D, et al. 2008, Nature 456:464-U22).
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| Contributor(s) |
Zhang J, Ye Y, Lin D |
| Citation(s) |
31015415 |
| BioProject |
PRJNA477937 |
| Submission date |
Jun 28, 2018 |
| Last update date |
May 02, 2019 |
| Contact name |
Liang Jia Zhang |
| E-mail(s) |
zjl1122@yeah.net
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| Organization name |
SYSUCC
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| Street address |
651 Dongfeng Road East, Yuexiu District,
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| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| SRA |
SRP151346 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116425_RAW.tar |
9.0 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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