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| Status |
Public on Sep 04, 2019 |
| Title |
Hypoxic transcriptomic signature of A549 cells (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To assess the transcriptomic response of the LUAD cell line A549 to hypoxia, cells were exposed to 1% (Hx) or 21% O2 (Nx) during 24 hours. Two µg of total RNAs were depleted from ribosomal RNA with the Ribozero kit (epicenter), libraries were generated with the NEBNext Small RNA Library Prep Set for SOLiD and sequenced on SOLiD 5500XL (Life Technologies) with single-end 50bp reads. Reads were aligned to the human genome release hg19 with the LifeScope software v2.5.1 (Life Technologies) using whole transcriptome pipeline for RNA‐seq libraries with default parameters.
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| |
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| Overall design |
2 samples in monoplicates: Nx versus Hx
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| |
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| Contributor(s) |
Rezzonico R, Nottet N, Mari B, Barbry P, Lebrigand K |
| Citation(s) |
31417181 |
| Submission date |
Jul 12, 2018 |
| Last update date |
Dec 04, 2019 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL16558 |
AB 5500 Genetic Analyzer (Homo sapiens) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE117049 |
The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress |
|
| Relations |
| BioProject |
PRJNA480905 |
| SRA |
SRP153245 |
