|
| Status |
Public on Oct 01, 2018 |
| Title |
Array-based genome-wide transcriptome analysis of IL-4-treated RAW264 macrohage cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The role of G-protein-coupled receptors (GPCRs) in the macrophage polarization remains not well understood. In this study, we illuminate the role of Gpr137b, a GPCR expressed strongly in macrophages, in macrophage polarization. We used RAW264 as a mouse macrophage-like cell line and generated Gpr137b-knockout (KO) cell clones using the CRISPR/Cas9 genome editing system. The Gpr137b-KO and wildtype cells were the treated with interleukin-4 (IL-4) to induce M2 polarization, and their gene expression was analyzed using microarrays.
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| |
|
| Overall design |
Gpr137b-knockout and wildtype Raw264 cells treated with or without IL-4.
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| |
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| Contributor(s) |
Islam Z, Ishibashi O, Horikawa A, Inui T |
| Citation(s) |
31372377 |
| Submission date |
Jul 24, 2018 |
| Last update date |
Aug 06, 2019 |
| Contact name |
OSAMU ISHIBASHI |
| E-mail(s) |
ishibashi@biochem.osakafu-u.ac.jp
|
| Phone |
+81-72-254-9474
|
| Organization name |
Osaka Prefecture University
|
| Department |
Grad Sch Life & Environmental Sciences
|
| Lab |
Biological Macromolecules
|
| Street address |
1-1 Gakuen-cho, Naka-ku
|
| City |
Sakai |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (8)
|
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| Relations |
| BioProject |
PRJNA482562 |
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