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| Status |
Public on Nov 30, 2008 |
| Title |
Human Skin: Before and 8 weeks after Isotretinoin Treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The pathogenesis of acne has been linked to multiple factors such as increased sebum production, inflammation, follicular hyperkeratinization, and the action of Propionibacterium acnes within the follicle. 13-cis Retinoic Acid (13-cis RA, isotretinoin) is the most potent agent in acne treatment. Surprisingly, its mechanism of action in acne is still unknown. Gene expression profiling of skin from 8 patients treated with isotretinoin was performed to gain insights into its mechanism of action. Skin biopsies were obtained from the patients at baseline and at 8 weeks isotretinoin treatment. Gene array expression profiling was conducted using Affymetrix HG-U133A 2.0 arrays in order to examine changes in gene expression as a result of treatment. After treatment, 784 genes were significantly changed: 197 up-regulated and 587 down-regulated. The majority of genes that were up-regulated at 8 weeks encode structural proteins of the extracellular matrix such as collagens, fibulin and fibronectin. The preponderance of genes that were down-regulated at 8 weeks are involved in the metabolism of steroids, cholesterol and fatty acids.
Keywords: drug treatment effects
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| |
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| Overall design |
Total 16 chips: 8 baseline/before isotretinoin and 8 after 8 weeks isotretinoin treatment.
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| |
|
| Contributor(s) |
Thiboutot DM |
| Citation(s) |
18987667, 20436886 |
| Submission date |
Jun 16, 2008 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Diane M Thiboutot |
| E-mail(s) |
dthiboutot@psu.edu
|
| Organization name |
The Pennsylvannia State University College of Medicine
|
| Department |
Department of Dermatology; Jake Gittlen Res. Fnd.
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA105993 |
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