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| Status |
Public on Aug 14, 2018 |
| Title |
AmpliSeq Transcriptome Analysis of Human Prostate Cancer Cells With or Without Overt SRRM4 Expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Prostate adenocarcinoma (AdPC) cells can undergo lineage switching to neuroendocrine cells and develop into therapy-resistant neuroendocrine prostate cancer (NEPC). While genomic/epigenetic alterations are shown to induce neuroendocrine differentiation via an intermediate stem-like state, RNA splicing factor SRRM4 can transform AdPC cells into NEPC xenografts through a direct neuroendocrine transdifferentiation mechanism. Whether SRRM4 can also regulate a stem-cell gene network for NEPC development remains unclear. Here, we use the Ion AmpliSeqTM Transcriptome Human Gene Expression Kit (AmpliSeq) to analyze the transcriptome of human AdPC cell line DU145 overexpressing SRRM4 via lentiviral transduction compared to the control, empty vector-transduced DU145 cells. This study reveals that SRRM4 induces a pluripotency gene network consisting of the stem-cell differentiation gene, SOX2. In summary, we report a novel mechanism by which SRRM4 drives NEPC progression via a pluripotency gene network.
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| Overall design |
Assessment of transcriptome profiles from DU145 cells with or without overt SRRM4 expression using AmpliSeq.
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| |
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| Contributor(s) |
Dong X, Lee AR, Gan Y |
| Citation(s) |
30100395 |
| Submission date |
Aug 03, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Xuesen Dong |
| E-mail(s) |
xdong@prostatecentre.com
|
| Organization name |
University of British Columbia
|
| Department |
Department of Urologic sciences, Vancouver Prostate Centre
|
| Lab |
Dong Lab
|
| Street address |
2660 Oak Street
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6H 3Z6 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA484414 |
| SRA |
SRP156322 |
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