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| Status |
Public on Dec 01, 2018 |
| Title |
Expression array of mammary epithelial cells isolated from epithelial Mmp14-targeted mammary glands |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mammary gland branching morphogenesis is thought to relie on the mobilization of the membrane-anchored matrix metalloproteinase, Mmp14/MT1-MMP, to drive mammary epithelial invasion by remodeling the extracellular matrix and triggering associated signaling cascades. However, the roles that this proteinase plays during postnatal mammary gland development in vivo remain undefined. A mammary gland branching program that occurs during the first 4 weeks of postnatal mouse development, in tandem with recently developed Mmp14-floxed mice and MMTV-Cre transgenics that express Cre recombinase throughout the mammary epithelial cell compartment, were used to characterize the impact of deleting epithelial cell Mmp14 on mammary gland morphogenesis.
Transcriptome profiling of mammary epithelial cells was used to investigate the functional roles of MT1-MMP in the postnatal mammary epithelial cell compartment in an unbiased fashion
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| Overall design |
Mammary epithelial cells were harvested from the mammary glands of conditional knockout [MMTV-Cre+/Mmp14(f/f)] mice and wild-type [Mmp14(f/f)] littermates on a congenic FVB/NJ background at 4 weeks after birth for RNA extraction and hybridization on Affymetrix microarrays.
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| |
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| Contributor(s) |
Feinberg TY, Weiss SJ |
| Citation(s) |
30269950 |
| Submission date |
Aug 13, 2018 |
| Last update date |
Mar 03, 2019 |
| Contact name |
Stephen J. Weiss |
| E-mail(s) |
sjweiss@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Life Sciences Institute
|
| Lab |
Stephen J. Weiss lab
|
| Street address |
210 Washtenaw Avenue
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (5)
|
| GSM3330565 |
MMTV-Cre+/Mmp14(f/f) mammary epithelial cells, biological replicate 1 |
| GSM3330566 |
Mmp14(f/f) mammary epithelial cells, biological replicate 1 |
| GSM3330567 |
Mmp14(f/f) mammary epithelial cells, biological replicate 2 |
| GSM3330568 |
Mmp14(f/f) mammary epithelial cells, biological replicate 3 |
| GSM3330569 |
MMTV-Cre+/Mmp14(f/f) mammary epithelial cells, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE118506 |
Affymetrix microarrays of mammary epithelial cells isolated from *Mmp14*-targeted mammary glands |
|
| Relations |
| BioProject |
PRJNA485735 |
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