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| Status |
Public on Aug 26, 2018 |
| Title |
A novel long non-coding RNA HOXC-AS3 mediates tumorigenesis of gastric cancer by binding to YBX1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Recently, increasing evidence shows that long noncoding RNAs (lncRNAs) play a significant role in human tumorigenesis. However, the function of lncRNAs in human gastric cancer remains largely unknown. By using publicly available expression profiling data from gastric cancer and integrating bioinformatics analyses, we screen and identify a novel lncRNA, HOXC-AS3. HOXC-AS3 is significantly increased in gastric cancer tissues and is correlated with clinical outcomes of gastric cancer. In addition, HOXC-AS3 regulates cell proliferation and migration both in vitro and in vivo. RNA-seq analysis reveals that HOXC-AS3 knockdown preferentially affects genes that are linked to proliferation and migration. Mechanistically, we find that HOXC-AS3 is obviously activated by gain of H3K4me3 and H3K27ac, both in cells and in tissues. RNA pull down-mass spectrometry analysis identifies that YBX1 interacts with HOXC-AS3, and RNA-seq analysis finds a marked overlap in genes differentially expressed after YBX1 knockdown and those transcriptionally regulated by HOXC-AS3, suggesting that YBX1 participates in HOXC-AS3-mediated gene transcriptional regulation in the tumorigenesis of gastric cancer.Together, our data demonstrate that abnormal histone modification-activated HOXC-AS3 may play important roles in gastric cancer oncogenesis and may serve as a target for gastric cancer diagnosis and therapy.
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| Overall design |
RNA-seq analysis of HOXC-AS3 and YBX1 knockdown in one cell type
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| |
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| Contributor(s) |
Zhang E, Yin D, Han L, De W |
| Citation(s) |
30286788 |
| Submission date |
Aug 24, 2018 |
| Last update date |
May 09, 2019 |
| Contact name |
wei de |
| E-mail(s) |
dewei_njmu@sina.com
|
| Phone |
+862586862728
|
| Organization name |
Nanjing Medical University
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
longmian da dao
|
| City |
nanjing |
| State/province |
jiangsu |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA487783 |
| SRA |
SRP158772 |
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