|
| Status |
Public on Oct 11, 2021 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Control and beta-catenin Deficient Cancer-associated Fibroblasts Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
D4M BrafV600E; Ptenlox5/lox5 melanoma cells mixed with uninduced control Fb (Ctnnb1+/+; Col1α2-CreER) or mutant bcat/Fb (Ctnnb1fl/fl; Col1α2-CreER) were injected intradermally into flanks of control Ctnnb1+/+; Col1α2-CreER or Ctnnb1 fl/fl; Col1α2-CreER mice, respectively. Tumors were allowed to grow to a volume of approximately 62.5 cubic millimeters when tamoxifen (TAM) was then administrated to induce β-catenin ablation in mutant bcat/Fb CAFs. Control CAFs or bcat/CAFs were collected from BrafV600E; Ptenlox5/lox5 melanoma tumors using PDGFRα as a marker for fibroblasts. Normal fibroblasts were collected from B6 mouse skin.
|
| |
|
| Overall design |
mRNA profiles of normal fibroblasts; control CAF and bcat/CAF were generated by Illumina HiSeq-based next generation sequencing in triplicate.
|
| |
|
| Contributor(s) |
Zhang Y, Zhou L, Liu T |
| Citation(s) |
35064015 |
| Submission date |
Oct 12, 2018 |
| Last update date |
Jan 25, 2022 |
| Contact name |
Yuhang Zhang |
| E-mail(s) |
yuhang.zhang@uc.edu
|
| Organization name |
University of Cincinnati
|
| Street address |
231 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA496035 |
| SRA |
SRP165595 |
Less...
More...