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| Status |
Public on Apr 16, 2020 |
| Title |
miRNA expression in breast cancer cell lines |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We analyzed the miRNA expression in 6 breast cancer cell lines from young (HCC1500, HCC1937) and old (MCF-7, MDA-MB-231, HCC1806 and MDA-MB-468) patients with breast cancer using the GeneChip® miRNA 2.0 Array (Affymetrix, Santa Clara, CA, USA).
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| Overall design |
Total RNA from BC cell lines was isolated in passage 4 using High Pure RNA Isolation Kit from Roche following the manufacturer’s protocol. RNA concentration was measured using a NanoDrop ND 2000-UV-vis Spectrophotometer (Thermo Fisher Scientific Inc., Wilmington DE, USA). RNA integrity determined by RIN (RNA integrity number) value was assessed with the Agilent 2100 Bioanalyzer using RNA 6000 Nano Assay (Agilent Technologies Inc., Santa Clara, CA, USA). Microarray expression profiling was performed using GeneChip® miRNA 2.0 Array (Affymetrix, Santa Clara, CA, USA).
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| Contributor(s) |
Oltra SS, Peña-Chilet M, Martinez MT, Tormo E, Climent J, Eroles P, Lluch A, Ribas G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 17, 2018 |
| Last update date |
Apr 18, 2020 |
| Contact name |
Sara OLTRA SANCHIS |
| E-mail(s) |
saraoltra4@gmail.com
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| Phone |
961973540
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| Organization name |
INCLIVA
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| Department |
Medical Oncology
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| Lab |
Cancer Functional Genomics
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| Street address |
Avda Menéndez Pelayo, 4 accesorio
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| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA497259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE121396_RAW.tar |
10.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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