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| Status |
Public on Apr 03, 2019 |
| Title |
Dysfunction and stemness of tumor-infiltrating T cells are triggered by a common mechanism |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The paradox of tumor immunology is that tumor infiltrating lymphocytes (TILs) are dysfunctional in situ and yet are capable of stem cell-like behavior with self-renewal, massive expansion, multipotency, and eradication of large metastatic tumors. Here we report that the overabundance of potassium in the tumor microenvironment (TME) can explain both phenomena.
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| Overall design |
Ctrl (or Samples labelled V- Vehicle) and elevated potassium (or Samples labelled K) were used to perfrom ChiP assays. CD8+ T cells from pmel mice were stimulated in vitro with 1 μM human gp100 peptide (10 μg/ml) in Ctrl (V) or elevated potassium (K) for 5 days and secondary re-stimulation was done for 48hrs with plate-bound anti-CD3 (1 μg/ml; BD Biosciences) and soluble anti-CD28 (1 μg/ml; BD Biosciences) and expanded in culture medium (Ctrl /K) containing 60 IU/mL of IL-2 for a total of 10 days.
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| Contributor(s) |
Vodnala SK |
| Citation(s) |
30923193 |
| Submission date |
Nov 05, 2018 |
| Last update date |
Apr 03, 2019 |
| Contact name |
Suman Kumar Vodnala |
| E-mail(s) |
suman.vodnala@nih.gov
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| Phone |
2408583809
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| Organization name |
NCI
|
| Department |
Surgery Branch
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| Lab |
Nicholas Restifo Lab
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| Street address |
10 Center Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (2) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA503845 |
| SRA |
SRP167854 |
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