|
| Status |
Public on Nov 20, 2018 |
| Title |
Telomere-Dependent and Telomere-Independent Roles of RAP1 in Regulating Human Stem Cell Homeostasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
RAP1 is well known as a telomere-binding protein; yet its functions in human stem cells remain unclear. Here we generated RAP1-deficient human embryonic stem cells (hESCs) by CRISPR/Cas9 and obtained RAP1-deficient human mesenchymal stem cells (hMSCs) and neural stem cells (hNSCs) via directed differentiation. In both hMSCs and hNSCs, RAP1 negatively regulated telomere length. In addition, RAP1 acted as a transcriptional regulator of RLEN by tuning the methylation status of its promoter region. Phenotypically, RAP1 deficiency resulted in enhanced self-renewal and delayed senescence in hMSCs rather than in hNSCs, suggesting a complex role of RAP1 in regulating lineage specific stem cell homeostasis. Altogether, these results indicate that RAP1 plays both telomeric and non-telomeric roles in regulating the homeostasis of human stem cells.
|
| |
|
| Overall design |
Transcriptomic and WGS analyses of WT and Rap1 deficiency mesenchymal stem cell or neural stem cells.
|
| |
|
| Contributor(s) |
Liu G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 17, 2018 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Zunpeng Liu |
| E-mail(s) |
zunpengliu@163.com
|
| Phone |
+86 15510715055
|
| Organization name |
State Key Laboratory of Stem Cell and Reproductive Biology
|
| Department |
Institute of Zoology, Chinese Academy of Sciences
|
| Lab |
Jing Qu
|
| Street address |
Beichen West Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA505881 |
| SRA |
SRP169501 |
Less...
More...