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| Status |
Public on May 01, 2019 |
| Title |
Expression array of Mt1-mmp+/+ and Mt1-mmp-/- mouse macrophages in response to LPS or IL-4 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Macrophages dominate inflammatory environments where they modify the extracellular matrix by mobilizing complex repertoires of proteolytic enzymes. Nevertheless, the dominant proteinases used by macrophage as they confront physiologic tissue barriers remain undefined. Herein, we have characterized the molecular mechanisms that define human macrophage-extracellular matrix interactions ex vivo. Resting and immune-polarized macrophages are shown to proteolytically remodel basement membranes while infiltrating the underlying interstitial matrix. In an unbiased screen to identify key proteases, we find that the macrophage metalloproteinase, MT1-MMP, is the dominant effector of basement membrane degradation and invasion. These studies not only identify MT1-MMP as a key proteolytic effector of extracellular matrix remodeling by human macrophages, but also define the invasive strategies used by macrophages to traverse physiologic tissue barriers.
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| Overall design |
Primary mouse bone marrow derived macrophages from global knockout [Mt1-mmp(-/-)] mice and wild-type [Mt1-mmp(+/+)] littermates on an outbred Swiss Black background were cultured for 24 hours in the presence of growth media alone or supplemented with 1ug/ml LPS or 20ng/ml IL-4 and harvested for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Bahr JC, Weiss SJ |
| Citation(s) |
36302921 |
| Submission date |
Nov 21, 2018 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Stephen J. Weiss |
| E-mail(s) |
sjweiss@umich.edu
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| Organization name |
University of Michigan
|
| Department |
Life Sciences Institute
|
| Lab |
Stephen J. Weiss lab
|
| Street address |
210 Washtenaw Avenue
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (11)
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| GSM3486180 |
Mt1-mmp(+/+) macrophage_untreated_biological rep1 |
| GSM3486181 |
Mt1-mmp(-/-) macrophage_untreated_biological rep1 |
| GSM3486182 |
Mt1-mmp(-/-) macrophage_untreated_biological rep2 |
| GSM3486183 |
Mt1-mmp(+/+) macrophage_LPS treated_biological rep1 |
| GSM3486184 |
Mt1-mmp(+/+) macrophage_LPS treated_biological rep2 |
| GSM3486185 |
Mt1-mmp(-/-) macrophage_LPS treated_biological rep1 |
| GSM3486186 |
Mt1-mmp(-/-) macrophage_LPS treated_biological rep2 |
| GSM3486187 |
Mt1-mmp(+/+) macrophage_IL4 treated_biological rep1 |
| GSM3486188 |
Mt1-mmp(+/+) macrophage_IL4 treated_biological rep2 |
| GSM3486189 |
Mt1-mmp(-/-) macrophage_IL4 treated_biological rep1 |
| GSM3486190 |
Mt1-mmp(-/-) macrophage_IL4 treated_biological rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE122825 |
Expression array of Mt1-mmp+/+ and Mt1-mmp-/- mouse macrophages and human macrophages in the presence or absence of MMP inhibitors |
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| Relations |
| BioProject |
PRJNA506441 |
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