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| Status |
Public on Feb 05, 2019 |
| Title |
Expression data from freshly isolated mouse mammary luminal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The mammary luminal cell compartment is heterogeneous. In adult virgin mice, it contains clonogenic luminal progenitors and non-clonogenic luminal cells expressing estrogen and progesterone receptors that can be
discrimated by their cell surface expression of ICAM-1. We have separated ICAM1+ luminal progenitors and ICAM- non-clonogenic luminal cells and analyzed their transcriptomic profiles. After showing that the luminal progenitor population overexpressed Met, Trp53 and numerous p53 target genes, we analyzed how the p53 and Met signaling pathways cooperate to control the function and plasticity of luminal progenitors.
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| Overall design |
Single cells were prepared from seven distinct pools of at least 12 mammary glands and sorted by flow cytometry according to their CD24 and ICAM-1 expression. Seven paired samples of CD24+ ICAM1+ (LuPos) and CD24+ ICAM1- (LuNeg) cells were collected for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Di-Cicco A, Bresson L, Rapinat A, Gentien D, de la Grange P, Faraldo MM, Deugnier M |
| Citation(s) |
30683141 |
| Submission date |
Nov 26, 2018 |
| Last update date |
Feb 05, 2019 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
|
| Organization name |
GenoSplice technology
|
| Department |
Paris Biotech Santé
|
| Street address |
29, rue du Faubourg Saint-Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA506953 |
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