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| Status |
Public on Sep 12, 2019 |
| Title |
SIGNAL INTEGRATION AND TRANSCRIPTIONAL REGULATION OF THE INFLAMMATORY RESPONSE MEDIATED BY THE GM-/M-CSF SIGNALING AXIS IN HUMAN MONOCYTES [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In recent years, the M-CSF and GM-CSF cytokines have been identified as opposing regulators of the inflammatory program. However, the two cytokines are simultaneously present in the inflammatory milieu, and it is not clear how cells integrate these signals. In order to understand the regulatory networks associated with the GM/M-CSF signaling axis we analyzed DNA methylation in human monocytes. Our results indicate that GM-CSF induces activation of the inflammatory program and extensive DNA methylation changes, while M-CSF-polarized cells are in a less differentiated state. This inflammatory program is mediated via JAK2 associated with the GM-CSF receptor and the downstream ERK signaling. However, PI3K signaling is associated with a negative regulatory loop of the inflammatory program and M-CSF autocrine signaling in GM-CSF-polarized monocytes. Our findings describe the regulatory networks associated with the GM/M-CSF signaling axis and how they contribute to the establishment of the inflammatory program associated with monocyte activation.
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| Overall design |
Two replicates were used per condition. Each sample was obtained from one individual. One replicate from the Trametinib sample group was excluded due to RNA degradation.
The RNA-seq samples and the ATAC-seq samples (GSE123569) are from the same biological samples.
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| Contributor(s) |
Rodriguez RM, Suarez-Alvarez B, Lavín JL, Ascensión AM, González M, Lozano JJ, Raneros A, Bulnes PD, Huidobro C, Martin-Martin C, Puig-Kröger A, Corbí AL, Araúzo-Bravo MJ, Aransay AM, Lopez-Larrea C, Vidal-Castiñeira JR, Sanz AB, Ruiz-Ortega M |
| Citation(s) |
31644909 |
| Submission date |
Dec 10, 2018 |
| Last update date |
Dec 12, 2019 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
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| Phone |
0034944061325
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| Organization name |
CIC bioGUNE
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| Department |
Genome Analysis Platform
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| Street address |
Parque tecnologico de Bizkaia, Building 801-A
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| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE123574 |
SIGNAL INTEGRATION AND TRANSCRIPTIONAL REGULATION OF THE INFLAMMATORY RESPONSE MEDIATED BY THE GM-/M-CSF SIGNALING AXIS IN HUMAN MONOCYTES |
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| Relations |
| BioProject |
PRJNA509249 |
| SRA |
SRP173248 |
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