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| Status |
Public on Jan 01, 2020 |
| Title |
Gene expression data of human chord blood hematopoietic progenior cells subjected to myeloid differentiation and transduced with lncRNA HOXA10-AS |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of gene expression and cell fate decisions. We predicted HOXA10-AS as a novel non-coding regulator of hematopoiesis and leukemogenesis We overexpressed HOXA10-AS using a bidirectional vector construct, which resulted in impaired monocytic in vitro differentiation and myeloid colony-formation. In addition, shRNA, sgRNA and LNA-GapmeR mediated downregulation of HOXA10-AS resulted in reduced leukemic growth of KMT2A-r cell lines and patient blasts. (p≤0.05). Here we assess early effects of ectopic HOXA10-AS expression in human HSPC subjected to myeloid differentiation conditions.
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| Overall design |
Human CD34+ HSPC from cord blood were transduced with HOXA10-AS or a empty control vector. Transduced cells were subjected to myeloid differentiation conditions. CD11b+CD117-CD14- myeloid precursors were FACS sorted on day 5 of culture. We analyzed early gene expression changes upon ectopic HOXA10-AS expression using the Affymetrix Microarray Clariom™ S Pico Assay
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| Contributor(s) |
Schwarzer A, Klusmann J |
| Citation(s) |
31867596 |
| Submission date |
Dec 12, 2018 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Jan-Henning Klusmann |
| E-mail(s) |
jan-henning.klusmann@kgu.de
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| Organization name |
Goehte University Frankfurt
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| Department |
Pediatric Hematology and Oncology
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| Street address |
Theodor-Stern-Kai 7
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| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA509607 |
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