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| Status |
Public on Dec 31, 2019 |
| Title |
Functional stem cell aging dictated by skin tissue microenvironment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Organismal aging in mammals is manifested with architectural alteration and functional decline of multiple organs throughout the body. In aged skin, hairs are sparse, which has led to the hypothesis that the hair follicle stem cells (HFSCs) undergo epidermal differentiation during aging. Here, we employ single cell analysis to interrogate aging-related changes in the HFSCs. Unexpectedly, HFSCs maintain their lineage fidelity and show no signs of shifting to an epidermal fate. Despite maintaining lineage identity, HFSCs do show prevalent transcriptional changes in extracellular matrix genes. Of importance, these HFSC changes are accompanied by profound architectural perturbations in the aging stem cell niche. Upon surveying the dermis from young and aged skin, we also observe age-related changes in many non-epithelial cell types, including resident immune cells, sensory neurons, arrector pili muscles, and blood vessels – all of which have been previously associated with abilities to modulate hair follicle regeneration. Consistent with both intrinsic and extrinsic alterations in stem cell: niche communications, we find that in response to skin wounding, aged HFSCs repair the epidermis, but are defective in hair follicle regeneration. Intriguingly, whereas aged dermis cannot support young HFSCs, aged HFSCs can be rescued when supported by young dermis. Together, these findings favor a model where skin tissue microenvironment plays a dominant role in dictating the molecular properties and activities of HFSCs.
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| Overall design |
Young and aged murine hair follicle stem cells and those from interfollicular epidermis in the skin were purified and assayed for their genome-wide transcriptional (RNAseq) and open chromatin (ATACseq) landscape, and 10x genomics was used to survey single cell transcriptome in young and aged skin epithelium
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| Contributor(s) |
Ge Y, Fuchs E |
| Citation missing |
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| Submission date |
Jan 10, 2019 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Yejing Ge |
| E-mail(s) |
yge@rockefeller.edu
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| Organization name |
The Rockefeller University
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| Street address |
1230 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA514242 |
| SRA |
SRP179070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124901_RAW.tar |
276.2 Mb |
(http)(custom) |
TAR (of BED, MTX, TSV, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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