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| Status |
Public on Jan 22, 2019 |
| Title |
Anti-apoptotic regulation contributes successful nuclear reprogramming using cryopreserved oocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT)- mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery effects of melatonin on cryo-damage in cloned embryos. Development of SCNT embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation of 8 pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1, P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin, an agent that reduces apoptosis and ROS production, enhanced blastocyst formation rates in the SCNT-CROP group when compared with the melatonin-untreated group. Additionally, melatonin-treatment increased the derivation efficiency of pluripotent stem cells from cloned embryos using cryopreserved oocyte.
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| Overall design |
Here we perform RNA-Seq experiment in melatonin-treated mouse 2-cell and blastocyst embryos, respectively.
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| Contributor(s) |
Lee DR, Lee AR, Park CH, Hong K |
| Citation(s) |
30799275 |
| Submission date |
Jan 21, 2019 |
| Last update date |
Mar 23, 2019 |
| Contact name |
Chanhyeok Park |
| E-mail(s) |
chpark0729@gmail.com
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| Organization name |
Konkuk University
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| Department |
Stem cell and regeneration biotechnology
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| Lab |
Hong lab
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| Street address |
120, Neungdong-ro
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| City |
Seoul |
| ZIP/Postal code |
05066 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA516198 |
| SRA |
SRP181026 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125389_all_genes.fpkm_table.txt.gz |
502.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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