 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 21, 2021 |
| Title |
Transcription profiles of human dermal papilla cells cultured under 20%-O2 or 6%-O2 condition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We investigated the effect of low oxygen culture on the proliferation and hair inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were cultured in atmospheric/hyperoxia (20% O2), physiological/normoxia (6% O2), or hypoxia (1% O2) conditions, respectively. Proliferation of DPCs and DSCs was highest under normoxia. Hypoxia inhibited proliferation of DPCs but enhanced proliferation of DSCs. In DPCs, hypoxia down-regulated expression of hair inductive capacity-related genes, including BMP4, LEF1, SOX2, and VCAN, and normoxia up-regulated expression of ALP. In DSCs, both normoxia and hypoxia up-regulated SOX2 expression, and hypoxia down-regulated BMP4 expression. Microarray analysis revealed increased expression of pluripotency-related genes, including SPRY, NR0B1, MSX2, IFITM1, and DAZL, under hypoxia. In an in vivo hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In DPCs, normoxia allowed the most efficient induction of hair follicles. In DSCs, hypoxia allowed the most efficient induction and maturation of hair follicles. These results suggest that low oxygen culture enhances the proliferation and maintains functions of human DPCs and DSCs and could be used for skin engineering and clinical applications.
|
| |
|
| Overall design |
Human DPCs were cultured for four weeks under 20%-O2 or 6%-O2 condition. Gene expression profiles were compared between two culture conditions by microarray analysis.
|
| |
|
| Contributor(s) |
Kanayama K, Takada H, Yoshimura K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 21, 2019 |
| Last update date |
Jan 23, 2021 |
| Contact name |
Hitomi Takada |
| E-mail(s) |
htakada@bs.naist.jp
|
| Phone |
81-743-72-5642
|
| Organization name |
Nara Institute of Science and Technology
|
| Department |
Graduate School of Science and Technology
|
| Lab |
Stem Cell Technology
|
| Street address |
8916-5 Takayama-cho
|
| City |
Ikoma |
| State/province |
Nara |
| ZIP/Postal code |
630-0101 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA516225 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125401_RAW.tar |
6.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
