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| Status |
Public on Aug 27, 2019 |
| Title |
Analysis of local glucocorticoid receptor occupancy at glucocorticoid-repressed inflammatory enhancers in Beas-2B airway epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study combined ChIP-seq using two different GR antibodies and stable GR knockdown in Beas-2B airway epithelial cells to determine whether glucocorticoid-mediated repression of inflammatory enhancers requires local GR occupancy
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| Overall design |
Duplicate samples of Beas-2B cells transduced with lentiviral-shRNA against GR (shGR) or a control construct (shCtrl) were treated with vehicle (ethanol), TNF (20 ng/ml), dexamethasone (100 nM) or TNF+dex for one hour prior to chromatin immunoprecipitation with one of two GR antibodies and sequencing. Duplicate Input DNA samples, representing both shCtrl and shGR groups, were included as controls.
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| Contributor(s) |
Sasse SK, Gruca M, Allen MA, Kadiyala V, Song T, Gally F, Gupta A, Pufall MA, Dowell RD, Gerber AN |
| Citation(s) |
31519741 |
| Submission date |
Jan 24, 2019 |
| Last update date |
Nov 26, 2019 |
| Contact name |
Sarah Sasse |
| Organization name |
National Jewish Health
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| Department |
Medicine
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| Lab |
Anthony Gerber
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| Street address |
1400 Jackson St., K720
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| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80206 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA516938 |
| SRA |
SRP181951 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125623_RAW.tar |
11.4 Gb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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