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| Status |
Public on Jan 29, 2019 |
| Title |
Fusion of reprogramming factors alters trajectory of somatic lineage conversion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to the transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce a transient acquisition of pluripotency before establishing the neural fate. We employed genetic lineage tracing and found that induction of iNSCs with individual vectors leads to direct lineage conversion. In contrast, polycistronic expression produces a Brn4-Klf4 fusion protein that enables the induction of pluripotency. Our study demonstrates that a combination of pluripotency and tissue-specific factors allows for direct somatic cell transdifferentiation, bypassing the acquisition of a pluripotent state. This result has major implications for lineage conversion technologies, which hold potential for providing a safer alternative to iPSCs for clinical application both in vitro and in vivo.
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| Overall design |
Here we perform RNA-Seq experiments in induced neural stem cells.
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| Contributor(s) |
Velychko S, Kang K, Park C, Schöler HR, Han DW |
| Citation(s) |
30943410 |
| Submission date |
Jan 28, 2019 |
| Last update date |
Apr 09, 2019 |
| Contact name |
Chanhyeok Park |
| E-mail(s) |
chpark0729@gmail.com
|
| Organization name |
Konkuk University
|
| Department |
Stem cell and regeneration biotechnology
|
| Lab |
Hong lab
|
| Street address |
120, Neungdong-ro
|
| City |
Seoul |
| ZIP/Postal code |
05066 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA517430 |
| SRA |
SRP182659 |
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