|
| Status |
Public on Jun 29, 2020 |
| Title |
Characterization of human erythroid chromatin in a two-phase liquid differentiation system |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
A two-phase liquid differentiation of peripheral blood mononuclear cells (PBMC) can be used to study human erythroid cells. To determine the epigenetic landscape of erythroid cells derived from PBMC we have performs ChIP-seq for histone marks associated with promoters (H3K4me3), enhancers (H3K4me1) and polycomb repression (H3K27me3) as well as for chromatin bound pol2. In addition we characterized the binding of the boundary element CTCF and key erythroid transcription factors NF-E2 and SCL/TAL.
|
| |
|
| Overall design |
Peripheral blood mononuclear cells were isolated and differentiated from a single male donor and a single replicate of ChIP-seq for histone marks (H3K4me1, H3K4me3, H3K27me3) and transcription factors (CTCF, NFE2, SCL/TAL, POL2). NG Capture-C was also performed on the same cells to determine the chromatin conformation of the alpha globin locus.
|
| |
|
| Contributor(s) |
Downes DJ, Roberts NA, Davies JO, de Gobbi M, Hughes JR |
| Citation(s) |
32518175 |
| Submission date |
Jan 28, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Damien Downes |
| E-mail(s) |
damien.downes@ndcls.ox.ac.uk
|
| Phone |
01865222374
|
| Organization name |
The University of Oxford
|
| Department |
MRC Weatherall Institute of Medicine
|
| Street address |
John Radcliffe Hospital
|
| City |
Headington |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA517452 |
| SRA |
SRP182670 |
Less...
More...