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| Status |
Public on Jul 18, 2020 |
| Title |
p53-inducible long non-coding RNAs encode functional peptides in hepatocellular carcinoma cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify p53-regulated lncRNAs responsive to DNA damage in human hepatocellular carcinoma (HCC) cells, we conducted paired-end and strand-specific RNA-seq using HepG2 wild-type and p53-knockout (HepG2-KO-p53) cells, which were devoid of p53 protein, using the CRISPR-Cas9 system treated with or without the DNA-damaging agent adriamycin (ADR)
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| Overall design |
RNA-seq profiles of HepG2 p53 wild-type (WT) and HepG2 p53 knockout (p53−/−) treated with ADR or not were generated by deep sequencing, in two independent experiments, using Illumina Xten. Raw RNA-seq data reads were aligned to the UCSC hg19 reference genome using Hisat2 (version 2.0.5) with default parameters.
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| Contributor(s) |
Xu W, Deng B, Lin P, Liu C, Li B, Zhou K, Zhou H, Qu L, Yang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 28, 2019 |
| Last update date |
Apr 01, 2022 |
| Contact name |
Jian-Hua Yang |
| E-mail(s) |
yangjh7@mail.sysu.edu.cn
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| Phone |
86-20-84112517
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| Organization name |
Sun Yat-sen University
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| Department |
School of Life Sciences
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| Street address |
No. 135, Xingang Xi Road
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| City |
Guangzhou |
| ZIP/Postal code |
510275 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE125758 |
p53-inducible long non-coding RNAs encode functional peptides in hepatocellular carcinoma cells |
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| Relations |
| BioProject |
PRJNA517459 |
| SRA |
SRP182675 |
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