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| Status |
Public on Aug 29, 2019 |
| Title |
Mip6 binds directly to the Mex67 UBA domain to maintain low levels of Msn2/4 stress dependent mRNAs |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
RNA binding proteins (RBPs) participate in almost all steps of gene expression, underscoring their potential as key regulators of RNA homeostasis. We structurally and functionally characterized Mip6, a four-RNA Recognition Motif (RRM)-containing RBP, as a functional and physical interactor of the mRNA export factor Mex67. Mip6 directly interacts with the ubiquitin-associated (UBA) domain of Mex67 through tryptophan 442 of Mip6-RRM4, whose mutation leads to slow growth of yeast cells in vivo. Although Mip6-RRM4 binds to RNA in vitro, Mex67 UBA binding prevents Mip6-RRM4 interaction with RNA. Mip6 shuttles between the nucleus and the cytoplasm in a Mex67-dependent manner and concentrates in cytoplasmic foci under several stressors. Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) experiments showed preferential binding of Mip6 to mRNAs regulated by the stress-response Msn2/4 transcription factors. Consistent with this binding, MIP6 deletion affected their export and expression levels. These results reveal a novel role for Mip6 in the homeostasis of Msn2/4 dependent transcripts by its direct interaction with Mex67 UBA domain.
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| Overall design |
Yeast Mip6 RNA binding at 30 ºC and after 39 ºC for 20 minutes using Illumina HI-Seq
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| Contributor(s) |
Martín-Expósito M, Merran J, Corden JL, de la Fuente L, Conesa A, Rodríguez-Navarro S |
| Citation(s) |
31680439 |
| Submission date |
Feb 07, 2019 |
| Last update date |
Nov 29, 2019 |
| Contact name |
Manuel Martín-Expósito |
| E-mail(s) |
manmartin89@gmail.com
|
| Organization name |
Instituto de Biomedicina de Valencia (IBV-CSIC)
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| Department |
Gene Expression and RNA Metabolism
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| Lab |
Rodríguez-Navarro Lab
|
| Street address |
Jaume Roig 11
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA521366 |
| SRA |
SRP184512 |
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