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| Status |
Public on Jan 28, 2020 |
| Title |
Differential response to Lenalidomide from WT and RUNX1-KO MDS-L del(5q) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used CRISPR/Cas9 system to generate WT Control and RUNX1-KO MDS-L cells isolated as single clones from the parental MDS-L cell line. We have observed that RUNX1-KO MDS-L cells are resistant to Lenalidomide-induced cell death compared to WT Control MDS-L cells
We have treated WT and RUNX1-KO MDS-L cells with 1µM Lenalidomide for 24h or 72h and performed expression analysis
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| Overall design |
The experiment consists in eight groups in total. Each group was analyzed with three technical replicates. The groups (per timepoint) are as follows: MDS-L WT DMSO; MDS-L WT LEN; MDS-L KO DMSO; MDS-L KO LEN. We then compared the transcriptional response to LEN within each genotype (MDS-L WT or MDS-L RUNX1-KO).Genes with padj<0.05 we identified as differentially expressed.
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| Contributor(s) |
Martinez-Hoyer S, Karsan A |
| Citation(s) |
32251398 |
| Submission date |
Feb 07, 2019 |
| Last update date |
Apr 28, 2020 |
| Contact name |
Aly Karsan |
| E-mail(s) |
akarsan@bcgsc.ca
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| Organization name |
BC Cancer Agency
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| Department |
Genome Sciences Centre
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| Street address |
W10th Ave
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA521440 |
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