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| Status |
Public on Feb 20, 2019 |
| Title |
Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1-/Δ7 mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The use of Akkermansia muciniphila as potential therapeutic intervention is receiving increasing attention. Health benefits attributed to this bacterium include an improvement of metabolic disorders and exerting anti-inflammatory effects. The abundance of A. muciniphila is associated with a healthy gut in early mid- and later life. However, the effects of A. muciniphila on a decline in intestinal health during the aging process are not investigated yet. We supplemented accelerated aging Ercc1-/Δ7 mice with A. muciniphila for 10 weeks and investigated histological, transcriptional and immunological aspects of intestinal health. The thickness of the colonic mucus layer increased about 3-fold after long-term A. muciniphila supplementation and was even significantly thicker compared to mice supplemented with Lactobacillus plantarum WCFS1. Colonic gene expression profiles pointed towards a decreased expression of genes and pathways related to inflammation and immune function, and suggested a decreased presence of B cells in colon. Total B cell frequencies in spleen and mesenteric lymph nodes were not altered after A. muciniphila supplementation. Mature and immature B cell frequencies in bone marrow were increased, whereas B cell precursors were unaffected. These findings implicate that B cell migration rather than production was affected by A. muciniphila supplementation. Gene expression profiles in ileum pointed toward a decrease in metabolic- and immune-related processes and antimicrobial peptide production after A. muciniphila supplementation. Besides, A. muciniphila decreased the frequency of activated CD80+CD273- B cells in Peyer’s patches. Additionally, the increased numbers of peritoneal resident macrophages and a decrease in Ly6Cint monocyte frequencies in spleen and mesenteric lymph nodes add evidence for the potentially anti-inflammatory properties of A. muciniphila. Altogether, we show that supplementation with A. muciniphila prevented the age-related decline in thickness of the colonic mucus layer and attenuated inflammation and immune-related processes at old age. This study implies that A. muciniphila supplementation can contribute to a promotion of healthy aging.
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| Overall design |
The effects of 10-wk bacterial supplementation of Akkermansia muciniphila on gut barrier and immunity was investigated in 16-week-old accelerated aging Ercc1-/Δ7 mice, which have a median lifespan of ~20wk. Distal ileum and proximal colon tissues were subjected to gene expression profiling.
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| Contributor(s) |
van der Lugt B, van Beek A, Aalvink S, Meijer B, Sovran B, Vermeij W, Brandt R, de Vos WM, Savelkoul H, Steegenga W, Belzer C |
| Citation(s) |
30899315 |
| Submission date |
Feb 19, 2019 |
| Last update date |
May 22, 2019 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (22)
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| GSM3611889 |
distal ileum of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 1 |
| GSM3611890 |
distal ileum of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 2 |
| GSM3611891 |
distal ileum of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 3 |
| GSM3611892 |
distal ileum of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 4 |
| GSM3611893 |
distal ileum of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 5 |
| GSM3611894 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 1 |
| GSM3611895 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 2 |
| GSM3611896 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 3 |
| GSM3611897 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 4 |
| GSM3611898 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 5 |
| GSM3611899 |
distal ileum of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 6 |
| GSM3611900 |
proximal colon of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 1 |
| GSM3611901 |
proximal colon of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 2 |
| GSM3611902 |
proximal colon of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 3 |
| GSM3611903 |
proximal colon of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 4 |
| GSM3611904 |
proximal colon of Ercc1-/∆7 mouse treated with vehicle (NaHCO3/PBS buffer), replicate 5 |
| GSM3611905 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 1 |
| GSM3611906 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 2 |
| GSM3611907 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 3 |
| GSM3611908 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 4 |
| GSM3611909 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 5 |
| GSM3611910 |
proximal colon of Ercc1-/∆7 mouse treated with Akkermansia muciniphila, replicate 6 |
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| Relations |
| BioProject |
PRJNA523132 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE126730_RAW.tar |
109.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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