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| Status |
Public on Oct 02, 2020 |
| Title |
Genome-wide maps of chromatin state in WT c-Myc and K427R mutant lymphoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in WT c-Myc and K427R mutant group. Inspection of multiple individual traces and global analyses showed that the K427R mutant globally reduced association of c-Myc with core promoters of its target genes.
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| Overall design |
we precipitated chromatin with tag antibody to WT c-Myc and K427R mutant, and sequenced the precipitated material. K427 mutation reduced association of c-Myc with core promoters of its target genes
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| Contributor(s) |
Hu Z, Li K, Wang F |
| Citation(s) |
33298911 |
| |
| Submission date |
Feb 19, 2019 |
| Last update date |
Dec 22, 2020 |
| Contact name |
wang feng |
| E-mail(s) |
wangfeng123@imm.ac.cn
|
| Phone |
15624968856
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| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College,
|
| Street address |
nan wei road
|
| City |
bei jing |
| ZIP/Postal code |
associate professor |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE126258 |
TRIB3 Promotes C-Myc-Associated Lymphomagenesis through Inhibition of UBE3B-Mediated c-Myc Degradation |
|
| Relations |
| BioProject |
PRJNA523183 |
| SRA |
SRP186264 |
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