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| Status |
Public on Jul 26, 2019 |
| Title |
Multiwalled carbon nanotube-induced blood genomic changes following aspiration exposure in mice: A 1-year postexposure study. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Pulmonary exposure to multiwalled carbon nanotubes (MWCNT) induces an inflammatory and rapid fibrotic response, although the long-term signaling mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in mice blood to identify non-invasive blood based biomarkers for medical and occupational surveillance.
The pathological results in this 1-year MWCNT post-exposure study was previously published in Snyder-Talkington et al. J Toxicol Environ Health A. 2016;79(8):352-66. doi: 10.1080/15287394.2016.1159635. Epub 2016 Apr 19. PMID: 27092743
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| Overall design |
We evaluated bronchoalveolar lavage (BAL) fluid for polymorphonuclear cell (PMN) infiltration and lactate dehydrogenase (LDH) activity, and lung histopathology for inflammatory and fibrotic responses in mouse lungs after 1 year post-exposure to a dispersion media (DM) control (Porter et al., 2008); 1, 10, 40, or 80 ug MWCNT; or 120 g crocidolite asbestos by pharyngeal aspiration. Additionally, we determined gene expression changes in mouse lung tissue with increasing doses of MWCNT at 1 year post-exposure to determine potential underlying mechanisms of fibrotic signaling after exposure to MWCNT.
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| Contributor(s) |
Guo NL |
| Citation(s) |
31146342 |
| Submission date |
Feb 22, 2019 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Nancy Lan Guo |
| E-mail(s) |
lguo@hsc.wvu.edu
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| Phone |
3042936455
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| Organization name |
West Virginia University
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| Department |
Occupational and Environmental Health Sciences
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| Lab |
Mary Babb Randolph Cancer Center/Guo Laboratory
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| Street address |
2816 Health Sciences Center
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| City |
Morgatown |
| State/province |
WV |
| ZIP/Postal code |
26506-9300 |
| Country |
USA |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (144)
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| Relations |
| BioProject |
PRJNA523853 |
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