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| Status |
Public on Feb 26, 2019 |
| Title |
PTENα/β paradoxically promote carcinogenesis through WDR5-H3K4me3 axis [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
we report that USP9X and FBXW11 selectively regulate the stability of PTENα/β but not PTEN proteins by deubiqitination and ubiquitination respectively. USP9X promotes and FBXW11 suppresses tumorigenesis mediated by PTENα/β. In contrast to the current paradigm for PTEN as a tumor suppressor, PTENα/β promote tumorigenesis of cancer cells in a phosphatase-independent manner. Mechanistically, PTENα/β localized in the nucleus regulate expressions of tumor-promoting genes such as NOTCH3 in the similar way as the H3K4 presenter WDR5. Further, PTENα/β but not PTEN directly interact with WDR5 to promote trimethylation of H3K4 and maintain a tumor-promoting signature.
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| Overall design |
RNA sequencing in parental and PTENα/βKO-1 SMMC-7721 cell lines with or without USP9X depletion by two independent experiments; RNA sequencing in MiaPaCa2 cell lines with or without PTENα/β
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| Contributor(s) |
Shen S, Zhang C, Ge M, Dong S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 24, 2019 |
| Last update date |
Feb 27, 2019 |
| Contact name |
ge mengkai |
| E-mail(s) |
gemengk@sjtu.edu.cn
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| Phone |
15201867201
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| Organization name |
Shanghai Jiao Tong University School of Medicine (SJTU-SM)
|
| Street address |
shanghai
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| City |
shanghai |
| State/province |
State... |
| ZIP/Postal code |
13185541251gmk |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE126984 |
PTENα/β paradoxically promote carcinogenesis through WDR5-H3K4me3 axis |
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| Relations |
| BioProject |
PRJNA524002 |
| SRA |
SRP186728 |
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