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Series GSE127116 Query DataSets for GSE127116
Status Public on Jun 11, 2019
Title Gene regulation by TLR2 and TLR10 in oncogene-induced senescence
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In the present study, we analyse the effect of knocking down TLR2 and TLR10 during oncogene induced senescence in IMR90 cells expressing a inducible version of oncogenic RAS (ER:RAS) in the transcriptome using Ampliseq RNA sequencig. We observed that TLR2 and TLR10 regulate the expression of many pro-inflammatory cytokines and chemokines that constitute the senescence associated secretory phenotype. There is also significant regulation of genes of the acute phase response.
 
Overall design IMR90 expressing ER:RAS fusion protein induced to senescence with activated oncogenic RAS by addition of 4 hydroxy-tamoxifen (4OHT), were transfected with siRNA to knockdown of TLR2 and TLR10. Total mRNA was extracted and expression profiles were analysed.
 
Contributor(s) Hari P, Acosta JC
Citation(s) 31183403
Submission date Nov 05, 2019
Last update date Nov 05, 2019
Contact name Juan Carlos Acosta
E-mail(s) juan-carlos.acosta@igmm.ed.ac.uk
Organization name The University of Edinburgh
Department Cancer Research UK - Edinburgh Centre
Street address Crewe Rd South
City Edinburgh
ZIP/Postal code EH4 2XR
Country United Kingdom
 
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (21)
GSM3625666 IMR90 ER:STOP siNon-Target 5 days IonXpress_001
GSM3625667 IMR90 ER:RAS siNon-Target 5 days IonXpress_002
GSM3625668 IMR90 ER:RAS siTLR2 5 days IonXpress_003
Relations
SRA SRP186796
BioProject PRJNA524181

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