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| Status |
Public on Jan 07, 2020 |
| Title |
FMRP cTag CLIP from mouse CA1 pyramidal neurons |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Loss of the neuronal RNA binding protein FMRP causes Fragile X Syndrome (FXS), the most common cause of inherited intellectual disability, yet it is unknown which brain regions and cell types within them contribute to disease pathophysiology. We used conditional tagging of FMRP and CLIP (cTag FMRP CLIP) to examine FMRP targets specifically in CA1 hippocampal neurons, a critical cell type for learning and memory known to have altered synaptic function in FXS. Integrating this data with analysis of ribosome-bound transcripts from the same neuronal population revealed CA1-enriched binding of autism-relevant mRNAs, and unexpected CA1-specific regulation of transcripts encoding circadian proteins.
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| Overall design |
3 biological replicates of FMRP cTag CLIP in mouse CA1 pyramidal neurons. Each replicate includes a sample prepared from Fmr1-cTag/Camk2a-Cre hippocampal tissue and a sample prepared from animals without Cre expression as a negative control.
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| Contributor(s) |
Sawicka K, Hale CR, Darnell RB, Darnell JC |
| Citation(s) |
31860442 |
| Submission date |
Mar 05, 2019 |
| Last update date |
Jan 07, 2020 |
| Contact name |
Kirsty Sawicka |
| E-mail(s) |
Kirsty.sawicka@cruk.cam.ac.uk
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| Organization name |
CRUK Cambridge Insititute
|
| Department |
University of Cambridge
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE127847 |
The Fragile X protein, FMRP, has a cell-type specific role in CA1 hippocampal neurons to regulate transcripts encoding autism-spectrum proteins and circadian memory |
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| Relations |
| BioProject |
PRJNA525639 |
| SRA |
SRP187542 |
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