|
| Status |
Public on Mar 08, 2020 |
| Title |
Genome-wide maps of chromatin state in liver cells. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse liver cells. We find that lysine 4 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 9 and 27 trimethylation marks primary coding and non-coding transcripts, have no differents in gene annotation. Trimethylation of lysine 4 is detected has different expresssion at mir124 site front of lnc-RNA(10835)Far. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations.
|
| |
|
| Overall design |
Examination of 3 different histone modifications in C57 and ficolinA ko mouse liver cells .
|
| |
|
| Contributor(s) |
Tang S, Long Q, Gao C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 08, 2019 |
| Last update date |
Mar 10, 2020 |
| Contact name |
Shu Li |
| E-mail(s) |
313135758@qq.com
|
| Organization name |
liver
|
| Department |
Department of Immunology
|
| Lab |
Xiao-Lian Zhang
|
| Street address |
Donghu Road 185
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430071 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA526252 |
| SRA |
SRP187980 |
Less...
More...