|
| Status |
Public on Mar 22, 2019 |
| Title |
Transcriptome of mature erythrocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Purpose: This study identifies the top transcripts expressed in mature erythrocytes and the top transcripts expressed in the polysome fractions of mature erythrocyte lysate
Methods: RNA from total erythrocyte lysate and polysome fractions was isolated and purified using GeneJet RNA purification column (Thermo Fisher Scientific). The RNA library was prepared using NEBNext ULTRA II RNA library preparation kit for Illumina. The libraries were sequenced using Illumina Seq.
Results: The transcriptome analysis revealed the abundance of mRNAs encoding globins genes, long non-coding RNAs, and micro RNAs. Deciphering the role and functions of these non-coding RNAs in these specialised cells could enable us to understand their biology in greater detail
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| Overall design |
RNA transcriptome profiling was done for total mature erythrocyte lysates and polysome fractions using Illumina Seq
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| |
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| Contributor(s) |
Eswarappa SM, Devi Kumar S |
| Citation missing |
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| Submission date |
Mar 21, 2019 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Sandeep Eswarappa |
| E-mail(s) |
sandeep@iisc.ac.in
|
| Organization name |
Indian Institute of Science
|
| Department |
Biochemistry
|
| Street address |
C V Raman Avenue, Devasandra layout
|
| City |
Bengaluru |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA528391 |
| SRA |
SRP189031 |
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