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| Status |
Public on Feb 26, 2020 |
| Title |
exosomal microRNA maps of HeLa cell exosome vs human cervical epithelial cell exosome [miRNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We aimed to compare the exosomal microRNA profile form HeLa cell line with that from human cervical epithelial cell(Hcer)line.exosomes were isolated from conditioned medium of HeLa cell and HCer cell,3 samples for each group.Exosomal microRNAs were sequenced on Proton Sequencers according to Ion PI Hi-Q Sequencing 200 Kit (Life Technologies).According to the results of miRNA sequencing, we found a total 371 different miRNAs the amount of which reach to 10 fold differece between two groups.
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| Overall design |
exosomal microRNA profiles of HeLa cell exosome vs human cervical epithelial cell (HCER) analysed using deep sequencing, in triplicate, using Illumina GAIIx.
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| Contributor(s) |
Yu H, Lin Y, Zhang C |
| Citation missing |
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| Submission date |
Mar 25, 2019 |
| Last update date |
Feb 26, 2020 |
| Contact name |
lin yinuo |
| E-mail(s) |
linyinuo@wmu.edu.cn
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| Organization name |
Second Affiliated Hospital of Zhejiang University
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| Street address |
Jiefang Road NO.88
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| City |
Hangzhou |
| ZIP/Postal code |
310009 |
| Country |
China |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA528920 |
| SRA |
SRP189345 |
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