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| Status |
Public on Mar 29, 2019 |
| Title |
Gene expression regulated by CSA and CSB in neuroblastoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mutations in the CSA and CSB genes are causative of Cockayne syndrome neurological disorder. Since the identification of the indispensable functions of these two proteins in transcription-coupled repair and restoring RNA synthesis following DNA damage, the paradoxical milder clinical spectrum in CS-A patients has been puzzling. In this study we compared the effect of a CSA and a CSB defect at the levels of the cell and the intact organism. We showed that CSA-deficient zebrafish embryos exhibited modest hypersensitive to UV damage than CSB depletion. We found that loss of CSA can effectively release aggregation of mutant crystallin proteins in vitro. We described the distinct effect of CSA and CSB on neuritogenesis and elucidated the differentiated gene expression pathways regulated by these two proteins. Our data demonstrate convergent and divergent roles for CSA and CSB in DNA repair and transcription regulation and provide explanations for the reported differences between CS-A and CS-B patients.
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| Overall design |
mRNA profiles of CSA-depleted, CSB-depleted and WT SH-SY5Y cells were generated by deep sequencing, using Illumina HiSeq X Ten in paired-end mode.
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| Contributor(s) |
Wu Z, Yu Q, Zhu X, Xu Y, Wang Y |
| Citation missing |
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| Submission date |
Mar 28, 2019 |
| Last update date |
Mar 30, 2019 |
| Contact name |
Yuming Wang |
| E-mail(s) |
wangyuming2006@hotmail.com
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| Phone |
008602037103555
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| Organization name |
Guangzhou medical university
|
| Department |
School of Basic Medical Science
|
| Street address |
Room 512, Anatomy Building
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
511436 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA529668 |
| SRA |
SRP189773 |
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