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| Status |
Public on Nov 30, 2019 |
| Title |
TTF-1/NKX2-1 and ASCL1 binding regions in a small cell lung carcinoma cell line, NCI-H209 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Although Thyroid transcription factor-1 (TTF-1, encoded by NKX2-1 gene) is highly expressed in small cell lung carcinoma (SCLC) and lung adenocarcinoma (LADC), difference in the functional roles of TTF-1 between SCLC and LADC remains to be elucidated. The aim of this study was to clarify the differences in the TTF-1 binding regions and functional roles in SCLC and LADC. Employing chromatin immunoprecipitation-sequencing (ChIP-seq) , here we compared the genome-wide TTF-1-binding profiles and the TTF-1-mediated transcriptional programs in a SCLC and a LADC cell lines. We also investigated ASCL1 binding regions in SCLC cells.
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| Overall design |
TTF-1 and ASCL1 ChIP-seq analysis was performed in a small cell lung carcinoma cell line, NCI-H209.
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| Contributor(s) |
Hokari S, Tamura Y, Morikawa M, Koinuma D, Miyazono K |
| Citation(s) |
31782890 |
| Submission date |
Apr 04, 2019 |
| Last update date |
Dec 03, 2019 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
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| Organization name |
University of Tokyo
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| Department |
Pathology
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| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE129341 |
Comparative Analysis of TTF-1 Binding DNA Regions in Small Cell Lung Cancer and Non-Small Cell Lung Cancer |
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| Relations |
| BioProject |
PRJNA530889 |
| SRA |
SRP190807 |
