|
| Status |
Public on May 21, 2019 |
| Title |
The landscape of transcription initiation across latent and lytic KSHV genomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Precise promoter annotation is required for understanding the mechanistic basis of transcription initiation. Here we use RAMPAGE to profile genome wide KSHV transcription initiation events, which will provide basis for further virus transcripts expression studies.
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| |
|
| Overall design |
We performed RAMPAGE in iSLK.219 and TREx-BCBL1-RTA cell lines, samples were taken at latency and dox induced lytic activation for 12h, 24h, 48h, 72h and 96h.
|
| |
|
| Contributor(s) |
Ye X, Zhao Y, Karijolich J |
| Citation(s) |
31188901 |
| Submission date |
Apr 16, 2019 |
| Last update date |
Jul 16, 2019 |
| Contact name |
John Karijolich |
| E-mail(s) |
john.karijolich@vumc.org
|
| Organization name |
Vanderbilt University Medical Center
|
| Department |
PMI
|
| Lab |
John Karijolich Lab
|
| Street address |
1211 Medical Center Drive
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA533118 |
| SRA |
SRP192756 |
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