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| Status |
Public on Apr 19, 2022 |
| Title |
rBC2LCN-positive subpopulation of PC-3 cells exhibits cancer stem-like properties |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We found that PC-3 cells consisted of rBC2LCN-positive and -negative subpopulations. To examine a global gene expression comparison between the rBC2LCN-positive and -negative subpopulations, DNA microarray was performed.
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| Overall design |
Human prostate cancer cell line, PC-3 cells, was maintained in F-12K medium (ATCC, 30-2004) supplemented with 10 % FBS (GIBCO, 12483-020) and 1 % Penstrep (SIGMA, I0516-5ML) at 37 °C and 5 % CO2. PC-3 cells were separated into rBC2LCN-positive and -negative subpopulations by live cell sorting using HiLyte Fluor 647-conjugated rBC2LCN. The total mRNAs were extracted from rBC2LCN-positive and -negative subpopulations using ISOGEN (NIPPON GENE). DNA microarray was performed by a SurePrint G3 Human GE Microarray Kit, 8 x 60 K Ver. 1 (Agilent, G4851A).
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| Contributor(s) |
Mawaribuchi S, Ito Y |
| Citation missing |
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| Submission date |
Apr 19, 2019 |
| Last update date |
Apr 22, 2022 |
| Contact name |
Yuzuru Ito |
| Organization name |
National Institute of Advanced Industrial Science and Technology (AIST)
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| Department |
Biotechnology Research Institute for Drug Discovery
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| Street address |
Central 6, Higashi 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
3058566 |
| Country |
Japan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA533823 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130092_RAW.tar |
24.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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