|
| Status |
Public on Apr 26, 2019 |
| Title |
Microarray analysis of lung SPC/GFP+ EpCAM+ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We extracted RNA of sorted lung SPC/GFP+ EpCAM+ cells and performed microarray analyses. Total RNA was extracted from sorted Ep-CAMhigh/GFPhigh cells using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. The RNA integrity was examined on an Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA using the GeneChip WT PLUS Reagent Kit User Manual (Affymetrix/Thermo Fisher Scientific). Fragmented and labeled ss-cDNA were hybridized on a GeneChip Clariom S Array (Affymetrix/Thermo Fisher Scientific) (n =3/group).
We used microarrays to detail the global gene expression of SPC/GFP+ EpCAM+ cells from the control mice(G29-5_(Clariom_S_Mouse)) and the 3 months smoke mice(G29-6_(Clariom_S_Mouse)), and identified distinct classes of up-regulated genes during this process.
|
| |
|
| Overall design |
Lung SPC/GFP+ EpCAM+ cells from the control mice and the 3 months smoke mice were sortd, RNA extraction from these cells was hybridized on Affymetrix microarrays.
|
| |
|
| Contributor(s) |
Tstsumi A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 25, 2019 |
| Last update date |
Apr 28, 2019 |
| Contact name |
Akihiro Tsutsumi |
| Organization name |
Keio University
|
| Street address |
shinanomachi35
|
| City |
Tokyo shinjukuku |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (2) |
| GSM3736252 |
Gene expression after 3 months air exposure |
| GSM3736253 |
Gene expression after 3 months smoke exposure |
|
| Relations |
| BioProject |
PRJNA535509 |
