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| Status |
Public on Sep 24, 2019 |
| Title |
Effects of social isolation stress on miRNA expression in the bed nucleus of stria terminalis (BNST) in male and female rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: This study assessed putative sex differences in miRNA expression in the bed nucleus of the stria terminalis (BNST)—a sexually dimorphic brain region implicated in anxiety—of adult male and female rats that had been exposed throughout adolescence to social isolation (SI) stress.
Methods: Male and female Sprague-Dawley rats underwent SI during adolescence or remained grouped housed (GH) till adulthood. Small RNA sequencing was performed on tissue extracted from the anterodorsal BNST.
Results: SI compared to GH induced more anxiogenic-like effects in females compared to males and sex-dependent changes in miRNA expression in the BNST.
Conclusions: The current study shows that housing conditions (either SI or GH) during adolescence regulates miRNA expression in a sex-specific manner in the sexually dimorphic area of the adBNST
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| Overall design |
Small RNA sequencing results were generated from the anterodorsal bed nucleus of stria terminalis (adBNST) of male and female group housed (GH) and socially isolated (SI) rats.
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| Contributor(s) |
Mavrikaki M, Pantano L, Potter D, Rogers-Grazado M, Amr S, Chartoff E |
| Citation(s) |
31636537 |
| Submission date |
May 15, 2019 |
| Last update date |
Oct 28, 2019 |
| Contact name |
Lorena Pantano |
| E-mail(s) |
lpantano@iscb.org
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| Organization name |
Harvard Chan School of Public Health
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| Department |
Biostatistics
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| Lab |
Bioinformatic Core
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| Street address |
655 Huntington Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA543123 |
| SRA |
SRP198536 |
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