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Series GSE131608 Query DataSets for GSE131608
Status Public on Mar 23, 2020
Title H3K36 methyltransferase SETD2 regulates lymphoid and neural development [MIXCR]
Organism Mus musculus
Experiment type Other
Summary Complete Amplicon Sequencing of a 500bp V(D)J recombination products from proB cells sorted from control and Setd2KO mice to Vh558, Vh7183 and Vhq52 variable gene familes to JH4 joining gene fragment to determine CDR3
 
Overall design Genomic DNA from proB cells (B220+Cd43+IgM-CD25-) sorted from control and Setd2 knockout mice were isolated and subjected to V(D)J recombination PCR using a primer for either Vh558, Vh7183, or Vhq52 gene families and JH4 gene. This 500bp PCR fragment was extracted and subjected to NGS (single 150bp read of amplicon pair)
 
Contributor(s) Chu H, Hatton C
Citation(s) 32169821
Submission date May 22, 2019
Last update date Mar 23, 2020
Contact name Charlie Hatton
E-mail(s) Charles_Hatton@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Scott Armstrong/CPCT
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (32)
GSM3790871 Mouse 1432 Igheavy chain Variable family 558 amplicon
GSM3790872 Mouse 1464 Igheavy chain Variable family 558 amplicon
GSM3790873 Mouse 1485 Igheavy chain Variable family 558 amplicon
This SubSeries is part of SuperSeries:
GSE131690 H3K36 methyltransferase SETD2 regulates NHEJ during lymphoid and neural development
Relations
BioProject PRJNA544179
SRA SRP199170

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131608_RAW.tar 270.0 Kb (http)(custom) TAR (of TXT)
GSE131608_kept.cdr3s.csv.gz 94.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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