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| Status |
Public on Aug 20, 2020 |
| Title |
Sexually dimorphic crosstalk at the maternal-fetal interface |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The first trimester is a critical window of maternal-fetal communication for pregnancy. Using single cell RNA-sequencing to dissect placenta heterogeneity, we identified five major cell types (trophoblasts, stromal cells, hofbauer cells, antigen presenting cells and endothelial cells). We identified seven unique trophoblast subclusters, including new subtypes that transition into the terminal cell types, extra-villous trophoblasts and syncytiotrophoblasts. As fetal sex impacts pregnancy, we analyzed sex differences in each cell type and identified differences in immune cell function. TGFβ1, β-estradiol, and dihydrotestosterone emerge as upstream regulators of sexually dimorphic genes in a cell type specific manner. Thus, the fetal contribution at the maternal-fetal interface is cell and sex specific.
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| Overall design |
Examination of transcriptomic profiles at the single cell level in the primary tissues from first trimester human placenta. In total, 6 placental tissues were utilized, of which 3 were from female conceptions and 3 were from male conceptions.
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| Contributor(s) |
Pisarska MD, Sun T, Gonzalez TL, Deng N, DiPentino R, Clark EL, Lee B, Tang J, Wang Y, Stripp BR, Yao C, Tseng H, Karumanchi SA, Koeppel AF, Turner SD, Farber CR, Rich SS, Wang ET, Williams III J |
| Citation(s) |
32772088 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD074368 |
Adverse Outcomes of Assisted Reproductive Technologies: Genetics or Epigenetics? |
CEDARS-SINAI MEDICAL CENTER |
Margareta Pisarska |
| R01 HD091773 |
Noncoding RNA regulation of the human placental transcriptome among the sexes |
CEDARS-SINAI MEDICAL CENTER |
Margareta Pisarska |
| U01 EB026421 |
Non-Invasive Prenatal Diagnostics Based on Circulating Trophoblasts |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Margareta Pisarska |
| T32 DK007770 |
Training Program in Endocrinology, Diabetes, and Metabolism |
CEDARS-SINAI MEDICAL CENTER |
SHLOMO MELMED |
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| Submission date |
May 23, 2019 |
| Last update date |
Aug 20, 2020 |
| Contact name |
Margareta D Pisarska |
| E-mail(s) |
pisarskam@cshs.org
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| Phone |
3106148307
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| Organization name |
Cedars Sinai Medical Center
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| Department |
OB/GYN, Div REI
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| Lab |
Pisarska
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| Street address |
8700 Beverly Blvd
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE131875 |
Sexually dimorphic crosstalk at the maternal-fetal interface |
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| Relations |
| BioProject |
PRJNA544522 |
| SRA |
SRP199367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131696_6sample_Expr_raw.csv.gz |
20.0 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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