|
| Status |
Public on May 25, 2020 |
| Title |
The impact of Prp18p on splicing fidelity and efficiency in budding yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Fidelity of 3´-splice site (3´SS) selection by the spliceosome is critical for proper gene expression but is a daunting task considering the low complexity of the 3´SS consensus YAG. Here we show that loss of the splicing factor Prp18p in budding yeast activates a diverse array of alternative 3´SS at more than half of all introns. Some alternative sites highly diverge from the YAG consensus, demonstrating a critical role for Prp18p in promoting spliceosome fidelity. Usage of alternative 3´SS is determined by distance from the branchpoint, local RNA secondary structures, upstream poly(U) content, and adenosine enrichment in exons. The 3´SS fidelity function of Prp18p can be genetically uncoupled from its role in splicing efficiency and is promoted by interactions with Slu7p and Prp8p. Taken together, these results provide a comprehensive mechanism into how the spliceosome achieves specificity of 3´SS selection and how it prevents aberrant activation of non-canonical splice sites.
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| Overall design |
RNA sequencing of yeast strains with and without the second step splicing factor Prp18p in a nonsense-mediated decay mutant background
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| Contributor(s) |
Roy K |
| Citation(s) |
37956322 |
| Submission date |
May 27, 2019 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Kevin Roy |
| E-mail(s) |
kevinroy@ucla.edu
|
| Organization name |
UCLA
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Guillaume Chanfreau
|
| Street address |
607 Charles E. Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA544962 |
| SRA |
SRP199582 |
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