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| Status |
Public on Jun 03, 2019 |
| Title |
Gene expression in prostate cancer cells chronically exposed or not to pollutant Aldrin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
DU145 prostate cancer cells were exposed for 50 days to a very low and no-toxic concentration of Aldrin (1 mM).
We used microarrays to detect potential effects of Aldrin in gene expression under chronic exposure to Aldrin.
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| Overall design |
DU145 cells were seeded in a 6-well plate at density of 2x10e5 per well in the presence of 1uM of Aldrin to a final volume of 2ml of supplemented RPMI (final concentration of DMSO 0.01%). Cells were cultured in standard conditions and diluted every 3 days. Cultures were treated after every passage to complete the 50-day treatment.
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| Contributor(s) |
Bedia C, Dalmau N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 02, 2019 |
| Last update date |
Jun 05, 2019 |
| Contact name |
Carmen Bedia |
| E-mail(s) |
carmen.bedia@gmail.com
|
| Phone |
+33 685358856
|
| Organization name |
Institute of Environmental Assessment and Water Research (IDAEA-CSIC)
|
| Department |
Environmental Chemistry
|
| Lab |
1442
|
| Street address |
Jordi Girona 18-26
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08034 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (8)
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| GSM3841594 |
Cells treated with 1uM Aldrin-replicate 1 |
| GSM3841595 |
Cells treated with 1uM Aldrin-replicate 2 |
| GSM3841596 |
Cells treated with 1uM Aldrin-replicate 3 |
|
| Relations |
| BioProject |
PRJNA545825 |
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