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| Status |
Public on Jan 30, 2020 |
| Title |
Single cell RNA-sequencing of normal and fibrotic lungs in mouse and human |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed scRNA-seq of cells obtained with a protocol designed to capture all of the collagen-producing cells from normal and fibrotic mouse and human lungs.
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| Overall design |
We induced lung fibrosis by treating two Col1a1-GFP reporter mice with bleomycin, and harvested lungs on day 14 after treatment. Two untreated Col1a1-GFP mice were used as control. After tissue dissociation, GFP+ cells and GFP- cells were sorted, and their single cell libraries were generated using 10x Genomics 3' v2 system. For human samples, we sorted lineage (CD31, CD45, EPCAM, CD235a)- cells and all lung cells (CD235a-) from three normal, three IPF, and two scleroderma lungs.
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| Contributor(s) |
Tsukui T, Sun KH, Matthay MA, Wolters PJ, Sheppard D |
| Citation(s) |
32317643 |
| Submission date |
Jun 14, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Tatsuya Tsukui |
| E-mail(s) |
tatsuya.tsukui@ucsf.edu
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| Organization name |
UCSF
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| Department |
Medicine
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| Street address |
555 Mission Bay Blvd South, 282
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA548947 |
| SRA |
SRP201481 |
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