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| Status |
Public on Jun 01, 2009 |
| Title |
Expression analysis to identify inducible genes in T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression microarrays were employed to identify genes induced by phorbol ester and ionomycin stimulation of EL4 cells. EL4 is a murine T cell line. To identify induced genes that were independent of new protein synthesis cells were pre-treated with cycloheximide. This expression study was used in conjunction with histone acetylation ChIP-chip to determine if inducible genes had a specific histone acetylation profile and whether the acetylation profile differed for genes with different kinetics of induction.
Keywords: drug treatment comparison
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| Overall design |
Cells were pre-treated with either DMSO or cylcoheximide in DMSO, then they were stimulated with PMA and Ionomycin for either 0h or 4h. 3 biological replicates were used for each treatment-stimulation combination.
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| Contributor(s) |
Hardy K, Lim C, Shannon F, Ma L, Peng K |
| Citation(s) |
19807913, 26981393 |
| Submission date |
Oct 20, 2008 |
| Last update date |
Oct 08, 2019 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE13279 |
Defining the chromatin signature of inducible genes in T cells |
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| Relations |
| BioProject |
PRJNA114013 |
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