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| Status |
Public on Jun 01, 2021 |
| Title |
Response of synovial fibroblasts to TNFα and IL6/sIL6R |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Synovial fibroblasts (SF) are the main mesenchymal cell type constitutive in human synovial tissue. SF contribute to the homeostasis of normal joints by synthesizing extracellular matrix components and secreting the specific components of synovial fluid. SF are essential players in RA pathophysiology, they are the primary source of IL6 in the RA synovium contributing to perpetuate the inflammation in the joint.
We used microarrays analysis to characterize the effector pro-inflammatory response of SF to TNFα and IL6/sIL6R signaling.
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| Overall design |
Four SF lines from healthy donors stimulated for 24h with either TNFα (20ng/ml) or IL6 and sIL6R (20ng/ml each) was used to synthesize double-stranded cDNA (ds-cDNA) by using GeneChip 3’ IVT Pico Reagent Kit. The fragmented and labelled ds-cDNA was hybridized in Affymetrix GeneChip® PrimeView™ Human Gene Expression Array platform containing 49395 probesets.
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| Contributor(s) |
Valín Á, Del Rey MJ, Pablos JL |
| Citation missing |
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| Submission date |
Jun 17, 2019 |
| Last update date |
Jun 02, 2021 |
| Contact name |
Manuel J Del Rey |
| E-mail(s) |
ermanu3@yahoo.es
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| Phone |
0034 91 3908766
|
| Organization name |
Instituto de Investigación Hospital 12 de Octubre
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| Department |
Grupo de Enfermedades Inflamatorias y Autoinmunes
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| Street address |
Avda Córdoba s/n
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| City |
Madrid |
| ZIP/Postal code |
20041 |
| Country |
Spain |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA549243 |
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